Visium HD Spatial Gene Expression

Visium HD

Novogene의 공간 전사체학 서비스는 10X Genomics의 Visium HD WT 패널 유전자 발현 데이터를 기반으로 하며, 단일 세포 해상도로 전체 전사체의 공간적 유전자 발현을 제공합니다. 신선 동결 조직(OCT 내장)과 FFPE 조직 모두에 호환되며, 조직학적 영상으로 형태를 보존하고 CytAssist를 이용한 전사 캡처 프로브를 전달해 정확한 공간 바코딩을 보장합니다. 이를 통해 연구자들은 온전한 조직 구조 내에서 유전자 발현을 시각화하고 복잡한 세포 상호작용을 밝혀낼 수 있습니다.

조직 커버리지에 빈틈이 없고 슬라이드당 6.5 x 6.5mm 크기의 이중 캡처 영역을 갖춘 이 새로운 플랫폼은 FFPE 조직 분석을 초고해상도로 제공합니다. 10X Genomics의 이 프로브 기반 유전자 발현 프로파일링 기술은 FFPE 샘플에 특화되어 있으며, 인간의 약 18,000개 유전자와 쥐의 약 20,000개 유전자를 대상으로 하여 전체 전사체에 대한 정보를 제공합니다. Novogene은 또한 10X Visium CytAssist 플랫폼을 활용해 전사체 프로브를 유리 슬라이드에서 Visium 슬라이드의 캡처 영역으로 옮기는 과정을 지원함으로써, 잘못된 위치 지정 오류를 줄이고 공간적 정확성을 유지합니다.

노보진 Visium HD: 장점 & 특징

엔드투엔드 서비스

샘플 QC부터 NovaSeq X Plus 시퀀싱, 그리고 서브미션 바로 가능한 BI 결과물까지 완전한 워크플로우를 제공합니다

고품질 데이터

일관되고 신뢰할 수 있는 시퀀싱 성능을 위해 업계 표준 데이터와 철저한 품질 관리를 보장합니다

종합적 BI 지원

Space Ranger와 Seurat 기반 분석, 서브미션에 적합한 공간 인사이트 피겨 포함

광범위한 실험 경험

공간 프로파일링 응용을 위한 다양한 인간 및 쥐 조직에서 입증된 경험

CSS 시스템

프로젝트 추적, 데이터 접근, 보고 및 효율적인 팀 협업을 위한 온라인 포털

연구분야에서의 Visium HD 활용

공간 전사체학은 다음과 같은 연구 분야에서 연구자들을 지원해 왔습니다:

암 연구

Visium HD는 종양, 면역세포, 기질세포 등 다양한 세포 유형이 종양 내에서 어떻게 배열되어 있는지 시각화해, 진행과 치료 반응에 영향을 미치는 공간적 패턴을 보여줍니다.

발생학

Visium HD는 발달생물학 연구를 위한 고해상도 다차원 공간 프로파일링 데이터를 제공하여 배아 발달, 장기 형성, 세포 운명 조절 메커니즘에 대한 깊은 이해를 돕습니다. 또한 정밀 육종과 질병 모델 구축 같은 응용 분야도 지원합니다.

신경 과학

뇌의 여러 영역과 층에 걸쳐 고해상도 유전자 발현 지도를 만들 수 있어, 연구자들이 뇌 기능, 발달, 그리고 알츠하이머 같은 신경학적 질환을 연구하는 데 도움을 줍니다.

감염 질환 및 면역 반응

Visium HD는 감염 시 면역 세포가 조직 내에서 어떻게 분포하고 활성화되는지 보여주어, 숙주-병원체 상호작용과 염증에 대한 통찰을 제공합니다.

연구분야에서의 Visium HD 활용

공간 전사체학은 다음과 같은 연구 분야에서 연구자들을 지원해 왔습니다:

암 연구

발생학

신경 과학

감염 질환 및 면역 반응

Visium HD는 감염 시 면역 세포가 조직 내에서 어떻게 분포하고 활성화되는지 보여주어, 숙주-병원체 상호작용과 염증에 대한 통찰을 제공합니다.

세부사항

샘플 준비방법

샘플 양은 참고용으로만 제시되어 있습니다. 자세한 내용은 샘플준비 가이드를 다운로드하여 확인하세요. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

세부사항

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

데이터 분석 예시

1/1

Bin 단위에서의 유전자 발현 공간 정량화

Space Ranger는 각 샘플의 모든 빈에서 각 유전자의 UMI를 계산하여 빈-유전자 발현 행렬을 만듭니다. 데이터 품질 관리를 수행한 후, 아래 그림은 조직의 공간 영역별로 유전자당 UMI 분포를 보여줍니다.

1/1

클러스터 분석: 공간 분포, tSNE 및 UMAP

Cluster_Spatial: 클러스터의 공간 분포 지도; Cluster_tSNE: 클러스터의 tSNE 2차원 공간 표현 지도; Cluster_UMAP: 클러스터의 2차원 공간 표현 지도

1/1

DEG의 t-SNE 및 UMAP 차원 축소 분석

각 클러스터에서 차등 발현된 상위 4개 유전자는 여러 가지 방식으로 표시됩니다. 이 그림은 차등 발현 유전자들의 t-SNE와 UMAP 차원 축소 시각화를 보여줍니다.

다른 서비스 탐색

Stereo-Seq Spatial

Single Cell Immune Profiling

Single Cell

프로젝트를 시작할 준비가 되셨나요?

문의 내용을 보내주시면 Novogene 전문가가 연락드리겠습니다.

Visium HD

노보진 Visium HD: 장점 & 특징

엔드투엔드 서비스

샘플 QC부터 NovaSeq X Plus 시퀀싱, 그리고 서브미션 바로 가능한 BI 결과물까지 완전한 워크플로우를 제공합니다

고품질 데이터

일관되고 신뢰할 수 있는 시퀀싱 성능을 위해 업계 표준 데이터와 철저한 품질 관리를 보장합니다

종합적 BI 지원

Space Ranger와 Seurat 기반 분석, 서브미션에 적합한 공간 인사이트 피겨 포함

광범위한 실험 경험

공간 프로파일링 응용을 위한 다양한 인간 및 쥐 조직에서 입증된 경험

CSS 시스템

프로젝트 추적, 데이터 접근, 보고 및 효율적인 팀 협업을 위한 온라인 포털

연구분야에서의 Visium HD 활용

공간 전사체학은 다음과 같은 연구 분야에서 연구자들을 지원해 왔습니다:

암 연구

발생학

신경 과학

감염 질환 및 면역 반응

Visium HD는 감염 시 면역 세포가 조직 내에서 어떻게 분포하고 활성화되는지 보여주어, 숙주-병원체 상호작용과 염증에 대한 통찰을 제공합니다.

연구분야에서의 Visium HD 활용

공간 전사체학은 다음과 같은 연구 분야에서 연구자들을 지원해 왔습니다:

암 연구

발생학

신경 과학

감염 질환 및 면역 반응

Visium HD는 감염 시 면역 세포가 조직 내에서 어떻게 분포하고 활성화되는지 보여주어, 숙주-병원체 상호작용과 염증에 대한 통찰을 제공합니다.

세부사항

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

세부사항

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

샘플 준비방법

Sample Type	Sample Amount	Preservation	Sample QC	Shipping
FFPE block (recommended)*	1 block, FFPE block must be contained on plastic dehydrating box, otherwise could not be installed on the sectioning device	After embedding store at 4°C, protected from light	DV200% ≥ 30%	Cold pack transportation
FFPE slide**	5-10 FFPE (10μm thickness) scrolls in tube for sample QC; 2-4 FFPE (5μm thickness) tissuesections on glass slides, for library prep*	Dry and sealed，storage time at 4°C< 14 days	DV200% ≥ 30%	Cold pack transportation
Fresh Frozen (OCT-embedded block)	Tissue thickness > 1mm FFPE blocks should be no older than 3 years *FFPE glass slides must be positive charged glass slides	After embedding store at -80°C , mark the cutting surface on the outside of the embedding box	RIN ≥ 4	Dry ice transportation

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

	Visium HD
Species	Human or mouse
Resolution	2 μm (single cell level)
Sequencing platform	NovaSeq X plus
Read length	PE150
Recommended Data Amount	FFPE: 100 Gb/sample; Fresh Frozen: 210 Gb/sample
Analysis	Data QC: Mapping and Quantification Dimensionality Reduction, Clustering, and Differential analysis: Graphclust clustering K-means clustering Seurat Analysis: PCA analysis Spatial variable gene expression analysis Clustering and dimensionality reduction analysis Differential Expression Gene Analysis (DEG): DEGs clustering heatmap Spatial distribution of DEGs Violine plot t-SNE and UMAP dimensional reduction visualization Functional Enrichment Analysis: GO enrichment KEGG enrichment Reactome enrichment

프로젝트 워크플로우

데이터 분석 예시

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Bin 단위에서의 유전자 발현 공간 정량화

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클러스터 분석: 공간 분포, tSNE 및 UMAP

Cluster_Spatial: 클러스터의 공간 분포 지도; Cluster_tSNE: 클러스터의 tSNE 2차원 공간 표현 지도; Cluster_UMAP: 클러스터의 2차원 공간 표현 지도

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DEG의 t-SNE 및 UMAP 차원 축소 분석

다른 서비스 탐색

Stereo-Seq Spatial

Single Cell Immune Profiling

Single Cell

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