Single Cell Gene Expression

Single Cell 시퀀싱 (scRNA-seq)

복잡한 집단 내 세포 이질성과 유전자 발현 동태를 밝히기 위한 고해상도 싱글셀 RNA-seq 프로파일링

Single Cell RNA 시퀀싱(scRNA-seq)은 bulk RNA 시퀀싱으로는 전혀 드러나지 않는 세포 간 차이와 세포 이질성을 감지할 수 있게 해줍니다. 개별 세포 수준에서 유전자 발현에 대한 고해상도 정보를 제공합니다. 생물학과 의학 전반에 걸쳐 폭넓게 활용되는 단일 세포 시퀀싱은 세포의 행동, 기능, 조절에 대한 전례 없는 이해를 제공합니다.

10x Genomics Chromium™ X 기술과 Illumina NovaSeq™ X Plus 플랫폼을 기반으로, Novogene은 샘플 처리와 라이브러리 준비부터 시퀀싱 및 생물정보학 분석까지 Single Cell RNA 시퀀싱(scRNA-seq)의 전 과정을 지원합니다. 깊이 있는 기술 전문성과 고객 중심의 접근 방식을 바탕으로, 저희 솔루션은 단일 세포 수준에서 전사체와 면역 프로파일링을 고해상도로 탐구할 수 있게 합니다.

노보진의 Single Cell 서비스: 장점 & 특징

엔드투엔드 서비스

샘플 처리부터 생물정보학, 그리고 출판 준비가 완료된 결과물까지 이어지는 원활한 scRNA-seq 워크플로우

검증된 전문성

업계 최고 수준의 신속한 결과와 까다로운 시료 유형에 대한 풍부한 경험을 자랑합니다

향상된 샘플 처리

신뢰할 수 있는 데이터 품질을 위한 핵 추출 및 특수 냉동 조직 작업 흐름을 포함한 유연한 처리

공인 우수성

높은 재현성, 민감도, 효율성을 위해 GEM-X & Chromium X를 사용하는 10X Genomics사의 공식 CSP입니다.

비용 효율적인 솔루션

최첨단 고처리량 플랫폼과 전문가 지원으로 경쟁력 있는 가격에 고품질 데이터를 제공합니다

연구 및 의학 분야에서의 Single Cell 활용

scRNA-seq는 다음과 같은 다양한 연구 분야를 지원하는 강력한 도구입니다:

암 연구

종양 미세환경 내 세포 이질성을 밝혀내어 암의 기전과 잠재적 치료 표적을 규명하는 것.

면역학

면역 세포 다양성을 지도화하고 면역 조절 및 기능 장애의 분자 메커니즘을 밝히다.

신경 과학

신경계 질환의 잠재적 치료 표적을 찾기 위해 발달 및 조절 네트워크를 해독합니다.

발생학

세포 계통과 발달 경로를 도식화하여 장기 형성과 조직 분화를 밝히는 연구.

세부사항

샘플 준비방법

샘플 양은 참고용으로만 제시되어 있습니다. 자세한 내용은 샘플준비 가이드를 다운로드하여 확인하세요. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

고객이 최고 품질의 데이터를 받을 수 있도록 전 과정에 걸친 서비스를 지원합니다. 여기에는 실험 설계, 샘플 준비, 라이브러리 구축, 시퀀싱, 생물정보학 분석 등 전 과정에 걸친 사전 전문가 협의가 포함됩니다.

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

세부사항

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

데이터 분석 예시

1/2

세포 클러스터

T-분포 확률적 이웃 임베딩(t-SNE)과 균일 다양체 근사 및 투영(UMAP)을 이용한 세포 군집 시각화. t-SNE와 비교했을 때, UMAP 시각화는 더 조밀한 시각적 군집을 가지며 그 사이에 빈 공간이 더 많습니다.

참고: 각 점은 하나의 셀을 나타내며, 각 색상은 하나의 클러스터를 의미합니다.

1/1

마커 유전자 히트맵

히트맵은 특정 세포와 특징에 대해 표시된 마커 유전자의 발현을 보여줍니다. 이번에는 각 군집별로 상위 10개 마커(10개 미만일 경우 모든 마커)를 그래프로 나타냈습니다.

참고: 진한 빨간색은 높은 발현 수준을, 진한 파란색은 낮은 발현 수준을 나타냅니다.

1/6

Violin Plot (마커 유전자)

바이올린 플롯은 모든 클러스터 내에서 마커 유전자의 상대적 발현 수준을 보여줍니다. (여기에는 여섯 개의 마커 유전자만 표시됩니다).

참고: X축은 클러스터를, Y축은 발현 수준을 나타냅니다.

다른 서비스 탐색

Visium HD

Single Cell Immune Profiling

Single Cell Long Read

프로젝트를 시작할 준비가 되셨나요?

문의 내용을 보내주시면 Novogene 전문가가 연락드리겠습니다.

Single Cell 시퀀싱 (scRNA-seq)

복잡한 집단 내 세포 이질성과 유전자 발현 동태를 밝히기 위한 고해상도 싱글셀 RNA-seq 프로파일링

노보진의 Single Cell 서비스: 장점 & 특징

엔드투엔드 서비스

샘플 처리부터 생물정보학, 그리고 출판 준비가 완료된 결과물까지 이어지는 원활한 scRNA-seq 워크플로우

검증된 전문성

업계 최고 수준의 신속한 결과와 까다로운 시료 유형에 대한 풍부한 경험을 자랑합니다

향상된 샘플 처리

신뢰할 수 있는 데이터 품질을 위한 핵 추출 및 특수 냉동 조직 작업 흐름을 포함한 유연한 처리

공인 우수성

높은 재현성, 민감도, 효율성을 위해 GEM-X & Chromium X를 사용하는 10X Genomics사의 공식 CSP입니다.

비용 효율적인 솔루션

최첨단 고처리량 플랫폼과 전문가 지원으로 경쟁력 있는 가격에 고품질 데이터를 제공합니다

연구 및 의학 분야에서의 Single Cell 활용

scRNA-seq는 다음과 같은 다양한 연구 분야를 지원하는 강력한 도구입니다:

암 연구

종양 미세환경 내 세포 이질성을 밝혀내어 암의 기전과 잠재적 치료 표적을 규명하는 것.

면역학

면역 세포 다양성을 지도화하고 면역 조절 및 기능 장애의 분자 메커니즘을 밝히다.

신경 과학

신경계 질환의 잠재적 치료 표적을 찾기 위해 발달 및 조절 네트워크를 해독합니다.

발생학

세포 계통과 발달 경로를 도식화하여 장기 형성과 조직 분화를 밝히는 연구.

세부사항

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

세부사항

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

샘플 준비방법

Service	Sample Type*	Sample Amount	Transportation Method
scRNA-seq	Cryopreserved cell suspension	Recommended Amount ≥1,000,000 cells per sample; (minimum ≥ 500,000 cells)	Needs to be shipped with dry ice
	Fresh cell suspension	Recommended Amount:300,000 cells per sample; (minimum ≥ 100,000 cells)	Needs to be shipped with ice packs within 1hr or harvest
	Frozen tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with dry ice
	Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

*Sample type preferences may vary based on sequencing requirements and sample compatibility.

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	20,000 – 50,000 read pairs/cell, 100-120Gb/sample
Analysis	Cell Ranger Analysis Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.

프로젝트 워크플로우

데이터 분석 예시

1/2

세포 클러스터

참고: 각 점은 하나의 셀을 나타내며, 각 색상은 하나의 클러스터를 의미합니다.

1/1

마커 유전자 히트맵

참고: 진한 빨간색은 높은 발현 수준을, 진한 파란색은 낮은 발현 수준을 나타냅니다.

1/6

Violin Plot (마커 유전자)

바이올린 플롯은 모든 클러스터 내에서 마커 유전자의 상대적 발현 수준을 보여줍니다. (여기에는 여섯 개의 마커 유전자만 표시됩니다).

참고: X축은 클러스터를, Y축은 발현 수준을 나타냅니다.

다른 서비스 탐색

Visium HD

Single Cell Immune Profiling

Single Cell Long Read

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