Single Cell Immune Profiling Seq 서비스

Single Cell Immune Profiling Seq

싱글셀 레솔루션에서 5' 유전자 발현과 함께 TCR, BCR 시퀀싱도 함께 분석하는 서비스

5′ GEX는 온전한 면역 세포와 비면역 세포 간의 전사 이질성을 포착하여 세포 유형, 활성 상태, 그리고 기능 프로그램을 식별하는 데 도움을 줍니다. 10X scRNA-seq 기반 단일 세포 RNA-seq 접근법은 복잡한 면역 집단 전반에 걸쳐 고해상도 단일 세포 유전자 발현 데이터를 생성합니다. T 세포 수용체(TCR)와 B 세포 수용체(BCR) 시퀀싱과 결합하면, 이 플랫폼은 유전자 발현과 면역 클론성을 동시에 분석할 수 있어 클론 확장과 면역 세포 표현형에 대한 통찰을 제공합니다.

노보진에서는 최신 10x Genomics Chromium iX 플랫폼과 GEM-X 화학 기반의 범용 5' 유전자 발현 분석법을 활용해, 높은 민감도와 재현성, 신뢰할 수 있는 성능을 갖춘 5′ GEX 및 면역 프로파일링 솔루션을 제공합니다. 저희의 효율적인 전 과정 워크플로우는 샘플 처리 및 품질 관리, 단일 세포 라이브러리 준비, NovaSeq X Plus를 이용한 고속 시퀀싱, 그리고 종합적인 생물정보학 분석을 포함합니다. 또한 직관적인 데이터 해석을 돕고 생물학적 발견을 가속화할 수 있도록, 출판에 적합한 명확한 시각화 자료도 제공합니다.

노보진 Single Cell Immune Profiling: 장점 & 특징

엔드투엔드 서비스

샘플 처리부터 시퀀싱, 생물정보학까지 매끄럽게 이어지는 작업 흐름과 출판 준비가 완료된 결과물

고품질 데이터

일관되고 신뢰할 수 있는 시퀀싱 성능을 위해 업계 표준 데이터와 철저한 품질 관리를 보장합니다

종합적 BI 지원

면역세포의 이질성과 기능을 밝혀내기 위한 Cell Ranger 파이프라인과 맞춤형 분석

비용 효율적 솔루션

경쟁력 있는 가격으로 고품질 데이터를 제공하는 고처리량 플랫폼과 전문가 지원

연구 및 의학 분야에서의 Single Cell Immune Profiling 활용

Single Cell 면역 프로파일링은 다음과 같은 연구 분야에서 면역 다양성 분석을 돕고 있습니다:

종양 미세환경

종양 면역 미세환경 특성과 면역치료 반응 기전 규명

감염성 질병

병원체 특이적 클론을 분석하여 감염 기전을 밝히고 항바이러스 치료의 근거를 제시한다

자가면역 질환

면역 레퍼토리의 클론 진화를 추적하여 비정상적으로 확장된 T세포 또는 B세포 하위 집단을 식별하고, 질병 아형 분류와 표적 발견에 도움을 줍니다

후보 백신 선별

백신 후보 검증을 가속화하기 위해 특정 TCR 또는 BCR을 신속하게 스크리닝합니다

세부사항

샘플 준비방법

샘플 양은 참고용으로만 제시되어 있습니다. 자세한 내용은 샘플준비 가이드를 다운로드하여 확인하세요. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

세부사항

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

샘플 준비방법

Service	Species*	Sample Type	Sample Amount	Transportation Method
5' GEX + TCR/BCR	Human/Mouse	Fresh single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Needs to be shipped with ice packs within 2hrs
		Frozen single cell suspension	Recommended Amount: > 1,000,000 cells per sample;	Ship with sufficient dry ice
		Fresh tissue	Tissue weight ≥ 200mg (minimum ≥ 100mg) ≥ 2 tissue pieces/sample, separate 1.5ml cryovials	Needs to be shipped with ice packs within 48hrs of harvest

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

시퀀싱 & 데이터 분석

권장 데이터 생산량과 표시되는 분석 내용은 참고용일 뿐입니다. 자세한 정보가 필요하시면 맞춤 요청을 가지고 저희에게 연락해주세요.

Sequencing Platform	Illumina NovaSeq X-Plus Sequencing Platform
Read Length	Paired-end 150 bp
Recommended Data	Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J
Analysis	Cell Ranger Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Summary metrics (sequencing quality, number of cells detected, the mean reads per cell, and the median genes detected per cell et al.). Alignment of reads to genome. Gene expression quantification. Clustering analysis. Differentially expression analysis between clusters. Visualization.	Standard Analysis (Gene Expression) Demultiplex BCL files from a sequencer into FASTQs. Alignment, UMI counting, Metrics summary. Identification of highly variable gene (HVGs). Cell Subpopulation Identification. Principal component analysis (PCA). Identify clusters of cells. Dimensionality reduction and Visualization. Marker gene detection (Differentially expression analysis between clusters). GO/KEGG/Reactome Enrichment. FunctionalAnnotation of Transcription Factor. Protein-Protein Interaction Network Analysis.	Standard Analysis (V(D)J) Demultiplex BCL files from a sequencer into FASTQs. Construction of Immune Repertoire(Alignment,Assembling contigs,Annotating contigs, Cell identification,Generating clonotype) Cell Filtration Clonotypes Statistics CDR3 Length Statistics Display of VJ Gene Abundance for Each Sample Clonotypes for Displaying VJ Gene Combinations Analysis of Highly Expressed Clonotypes by Multi-Sample Comparison Clone Proportion Analysis Clonal Diversity Analysis

데이터 분석 예시

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CDR3 길이 패턴

레퍼토리 특성화와 샘플 비교를 지원하기 위해 CDR3 길이 패턴을 검토합니다.

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클로노타입 수

샘플이나 그룹 간 고유 클론형의 수를 비교하여 면역 레퍼토리의 다양성을 탐구합니다.

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V 유전자 사용 패턴

샘플별 V 유전자 사용 패턴을 시각화하여 T 세포 집단 내 수용체 사용 차이를 파악합니다.

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Clonal Proportion

Compare the proportion of expanded and low-frequency clonotypes to assess clonal structure across samples.

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V-J Gene Pairing

Visualise V-J gene pairing relationships to support interpretation of receptor recombination and repertoire diversity.

다른 서비스 탐색

Single Cell (scRNA-seq)

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Single Cell Long Read

프로젝트를 시작할 준비가 되셨나요?

문의 내용을 보내주시면 Novogene 전문가가 연락드리겠습니다.

Single Cell Immune Profiling Seq

싱글셀 레솔루션에서 5' 유전자 발현과 함께 TCR, BCR 시퀀싱도 함께 분석하는 서비스